In an image pickup unit, a first optical system holding frame 21 and a second optical system holding frame 22 are set relatively movable in a direction orthogonal to an optical axis O of photographing light made incident on a first object lens group 41, 42, and the second optical system holding frame 22 and a third optical system holding frame 23 are set relatively movable in a direction along the optical axis O of the photographing light made incident on a solid-state image pickup device 51 such that predetermined optical performance adjustment can be performed during assembly.