The present invention relates to a method for producing de novo papillae, in which dermal hair papilla fibroblasts (DPFs) are isolated from a dermal papilla (DP) of a mammal hair follicle, subsequently expanded, and finally condensed into cell aggregates that exhibit the size and shape of the physiological DP, wherein said DPFs are differentiated in ultra-low attachment culture vessels in a cell concentration per vessel surface of 1.000 to 100.000 DPFs/cm 2 . The invention also relates to a method for producing hair microfollicles by co-culturing the de novo papillae with another cell population of the hair follicle in the ultra-low attachment culture vessels. Object of the invention are also the de novo papillae and the hair microfollicles produced by the aforementioned methods. The de novo papillae and hair microfollicles can be used as implants for treating reduced hair conditions, and for in vitro testing hair-modulating effects or toxic effects of substances.本発明は毛小嚢を作製する方法に関し、前記方法は(a)デノボ乳頭を提供する工程、(b)線維芽細胞、角化細胞及びメラニン細胞の群から選択される他の細胞集団を提供し、更に前記デノボ乳頭を少なくとも1つの他の細胞集団と一緒に非接着性培養容器で同時培養する工程を含む。本発明はまた、毛小嚢を作製する前記方法で使用することができるデノボ乳頭を作製する方法に関する。
