#$%^&*AU2010202610B220130110.pdf#####ABSTRACT: Isolated polynucleotide regulatory sequences from Lolium perenne (perennial ryegrass), Festuca arundinacea (tall fescue) and Arabidopsis thaliana that are involved in the regulation of gene expression are provided, together with methods for the use of such polynucleotide regulatory regions in modifying the expression of endogenous and/or heterologous polynucleotides in transgenic plants. In particular, the present invention provides polynucleotide promoter sequences from 5' untranslated, or non-coding, regions of plant genes that initiate and regulate transcription of polynucleotides placed under their control, together with isolated polinuc leotides comprising such promoter sequences.