WONG, KA LEUNG,黄 嘉良,黃 嘉良,LI, HONGGUANG,李红光,李紅光,CHAN, CHI FAI,陈 智辉,陳 智輝,LAN, RONGFENG,蓝荣峰,藍榮峰,黄 嘉良,黃 嘉良,陈 智辉,陳 智輝
申请号：
TW106117745
公开号：
TWI641601B
申请日:
2017.05.26
申请国别(地区):
TW
年份:
2018
代理人:
摘要：
The present invention relates to a water-soluble, simple, stable tris(N-(tert-butyl)acetamide) cyclen-based europium complexHGEu001which exhibits the specific subcellular localization in the primary cilium with a quantum yield as high as 10 % in water and a lifetime of 0.56 ms lifetime. In particular, the present invention provides simplicity of the design and synthesis of a complex. Comprehensive studies were performed in numerous cell lines, such as HeLa, SN-K-SH and MRC5； the motif structure,HGEu002, has also been synthesized as the negative control forin vitroimaging studies. The two-photonin vitroimaging were done in three dimensions to emphasize on the specific localization in primary cilium ofHGEu001. This is one of the very limited examples for direct primary cilium imaging.本發明係關於一種水溶性的簡單穩定的基於參(N-(第三丁基)乙醯胺)大環多胺之銪錯合物HGEu001，該銪錯合物展現出在初級纖毛中之特異性亞細胞定位，其中於水中之量子產率高達10%且生命期達0.56 ms之生命期。詳言之，本發明簡化錯合物之設計及合成。在諸如HeLa、SN-K-SH及MRC5之多種細胞株中執行全面研究；亦已合成基元結構HGEu002作為活體外成像研究之陰性對照。三維雙光子活體外成像已被完成以強調HGEu001在初級纖毛中之特異性定位。此情況係直接初級纖毛成像之極有限實例之一。
