ACELLULAR TREATMENT FOR MATERIAL DERIVED FROM BIOLOGICAL TISSUE, A METHOD FOR TREATING USING THEREOF, AND BIOLOGICAL MATERIAL OBTAINED FROM THIS METHOD
JANG, JI WOOK,장지욱,KIM, HYUN JUNG,김현정,LEE, SHI WOO,이시우,KIM, SE HOON,김세훈
申请号：
KR1020120041230
公开号：
KR1020130118418A
申请日:
2012.04.20
申请国别(地区):
KR
年份:
2013
代理人:
摘要：
PURPOSE: An acellular treatment formed of a material derived from biological tissues and a treatment method using the same are provided to stably remove cells, fats, viruses, and endotoxin through a treatment with a polar solvent, acids, and alkali. CONSTITUTION: An acellular treatment contains alkali and a polar solvent and is a primary remover of an immunity and neophobia inducer derived from a biological tissue. The alkali is selected among sodium hydroxide, potassium hydroxide, ammonium hydroxide, calcium carbonate, magnesium hydroxide, calcium hydroxide, and ammonia and is used in a concentration of 0.01-1 M. The polar solvent is selected among C1-C4 alcohols and is contained in a concentration of 10-100%. The alkali and the polar solvent are mixed in a ratio of 1:9-9:1. The material is treated by the acellular treatment. A biological material contains collagen and elastin. The thickness and the tensile strength of the biological material are 10 um to 3 mm and 0.01-20 MPa, respectively, for transplantation and procedure. The biological material has superior biocompatibility, cell affinity, and immunocompetence.본 발명은 생체조직 유래 소재의 탈세포 처리제, 이를 이용한 처리방법 및 이로부터 수득된 생체 재료에 관한 것으로, 생체조직 중 면역 및 이물반응 유발 물질인 세포, 유전자, 지방 및 바이러스와 엔도톡신을 효율적으로 제거 가능한 탈세포 처리제를 제공할 수 있으며, 이 같은 특정 탈세포 처리제로 처리함으로써 수득된 생체 재료가 생체적합성이 우수하고, 생체 이식에 적합한 강도를 가짐으로써 연조직 재건 및 조직수복 용도로 사용될 수 있다.
