TIMMERMAN, PETER,PUIJK, WOUTER, CORNELIS,MELOEN, ROBBERT, HANS
申请号：
NZ57442307
公开号：
NZ574423A
申请日:
2007.07.26
申请国别(地区):
NZ
年份:
2012
代理人:
摘要：
Disclosed is a method for inducing and/or enhancing immunogenic reproducibility and/or immunogenicity of an amino acid sequence, comprising a. coupling said amino acid sequence via at least two linkages at a site outside an epitope to a (hetero)aromatic molecule with at least two benzylic halogen substituents wherein each of the at least two linkages is formed by reacting a benzylic halogen substituent of the (hetero)aromatic molecule with a nucleophilic functionality within the peptide structure and b. forming at least one internal bond within said amino acid sequence at a site outside an epitope after coupling of the amino acid sequence to the (hetero)aromatic molecule, wherein the at least one internal bond is a disulphide bond or a diselenium bond. Further disclosed are immunogenic compounds comprising an amino acid sequence bound to a (hetero)aromatic molecule via at least two linkages at a site outside an epitope, wherein said at least two linkages are at a benzylic position of said (hetero)aromatic molecule, wherein said amino acid sequence comprises at least one internal bond, and wherein said internal bond is a disulphide bond or a diselenium bond.
