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Methods for cryopreservation of stem cells via slow-freezing
专利权人:
发明人:
Sven Comhaire,Björn Comhaire,Frank Eertmans,Veerle Bogaert
申请号:
US14574748
公开号:
US09458424B2
申请日:
2014.12.18
申请国别(地区):
US
年份:
2016
代理人:
摘要:
Cryopreserved stem cells with high cell viability after thawing were obtained by slow freezing using a DMSO-free cryopreservation medium. Stem cells and/or progenitor cells thereof are contacted with a DMSO-free cryopreservation medium, comprising between 4 v/v % and 25 v/v % of propylene glycol and between 1.0 w % and 10 w % of a sugar and are subsequently subject to a slow-freezing process. Optionally, the cryopreservation medium may comprise serum albumin and/or hyaluronic acid.
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