National University of Singapore;Singapore Health Services Pte Ltd
发明人:
Karl Tryggvason,Monica Suryana Tjin,Alvin Wen Choong Chua
申请号:
US16610971
公开号:
US20200071666A1
申请日:
2018.05.04
申请国别(地区):
US
年份:
2020
代理人:
摘要:
The present disclosure relates to methods for culturing human epidermal keratinocytes. When keratinocytes are cultured on plates coated with a laminin containing an alpha-4 chain or an alpha-5 chain, in a xeno-free, chemically defined cell culture medium, they expand efficiently in vitro. Useful cell culture kits for culturing keratinocytes are also described herein, as are methods of using such cells for treatment of burns or chronic wounds.