Disclosed is a method for removing endotoxin from proteins. Also disclosed areproducts made by using the method. The method may be used, for example, to produceendotoxin-free lactoferrin. Bovine milk-derived lactoferrin may be producedin commercial quantities by the method, and endotoxin-free bovine lactoferrinmay be used for a variety of therapeutic uses, including improving wound healing.