The present invention is related to a novel functional nanoparticle-based antibiotics and preparation method thereof, especially related to an antibiotics-modified nanoparticle. The functional nanoparticle-based antibiotics according to the present invention can be used as the affinity probes and the photothermal agents to effectively inhibit the cell growth of pathogenic bacteria under NIR irradiation.本發明係提供一種新穎之功能性奈米粒子抗生素及其製造方法,尤其是一種表面具有抗生素修飾之奈米粒子,以解決臨床上普遍存在之細菌抗藥性之問題。本發明之功能性奈米粒子探針能辨識致病細菌,在近紅外光波長照射下可用為光熱試劑,並有效地抑制細菌之生長。101...合成奈米粒子溶液102...製備具活性之抗生素溶液103...將奈米粒子溶液加入抗生素溶液反應104...製備本發明之功能性奈米粒子抗生素