The present Invention relates to the use of Lactobacillus rhamnosus in combination with Bifidobacterium longum in the preparation of a composition to increase Male Fertility in a subject. In particular, these strains are strains of Bifidobacterium and Lactobacillus rhamnosus CECT 8361 CECT 7347.It also describes the composition comprising Said strains Bifidobacterium and Lactobacillus rhamnosus CECT 8361 CECT 7347La presente invencion se refiere al uso de Lactobacillus rhamnosus en combinacion con Bifidobacterium longum en la elaboracion de una composicion para incrementar la fertilidad masculina en un sujeto. En particular, dichas cepas son las cepas de Lactobacillus rhamnosus CECT 8361 y Bifidobacterium longum CECT 7347. Asimismo, tambien se describe la composicion que comprende dichas cepas Lactobacillus rhamnosus CECT 8361 y Bifidobacterium longum CECT 7347