A device for retaining a biological sample for performing a cryoprocedure on a biological sample having a tubular member having a lumen extending therein, the lumen configured to receive the biological sample. A retainer is couplable to a distal end of the tubular member, the retainer having a perforated member having at least one orifice, the at least one orifice having a dimension smaller than a dimension of the at least one biological sample to prevent exit of the biological sample from the tubular member, wherein the perforated element is configured to allow inflow of liquids to communicate with the lumen containing the biological sample.