PURPOSE: A natural surfactant composition is provided to ensure excellent surface activity, foaming ability, washing ability, dispersion, and emulsification. CONSTITUTION: A natural surfactant composition contains a mixture extract of Polygala tenuifolia willd., Sophora flavescens aition, and Rosa spp. mixed in a weight ratio of 0.5-1.5:0.5-1.5:0.5-1.5. The extract is selected from the group consisting of water, ethanol, methanol, propanol, butanol, glycerol, propylene glycol, and 1,3-butylene glycol. A cosmetic composition or a washing composition contains the natural surfactant composition. [Reference numerals] (AA) Synthetic surfactant 47mm; (BB) Mixed extract 41mm본 발명은 원지, 고삼 및 장미의 혼합 추출물을 포함하는 것을 특징으로 하며, 인체에 무해하고 환경 안정성이 있으며 기포력, 세정력, 분산력 및 유화력이 매우 뛰어난 천연 계면활성제 조성물에 관한 것이다.