A method of processing a lipoaspirate sample includes mechanically processing the lipoaspirate sample to generate nanofat. The nanofat is then input into a microfluidic device comprising a plurality of serially arranged stages comprising one or more microfluidic channels having a plurality of expansion and constriction regions extending along the length of the one or more microfluidic channels, wherein each subsequent stage of the plurality has an increasing number of microfluidic channels of decreasing dimensions. The nanofat is flowed through the plurality of serially arranged stages in a plurality of cycles to generate sheared nanofat. The sheared nanofat is then collected after flowing through the plurality of serially arranged stages. The sheared nanofat may then be injected and/or applied to the subject. In an alternative embodiment, filtered or mechanically processed lipoaspirate may be run through the microfluidic device.
