A liquid ejection device that suppresses scatter of the living tissue, such as cells excised, fragmentated, or otherwise separated by ejected pulsed flow is provided.The liquid ejection device ejects, through a nozzle 4, first liquid 12 in the form of pulses supplied through an ejection tube 3 and includes a supply tube 23 , through which second liquid 31 is supplied to a region in the vicinity of the nozzle 4. The liquid ejection device further includes a suction port 15, which is located in the vicinity of the nozzle 4, and a suction pump 18, which is connected to the suction port 15 via a suction tube 14 and sucks the first liquid 12 and the second liquid 31.