A radiograph analysis device is configured to detect a marker area from a radiograph obtained by imaging a specimen in whichh a marker is embedded, and the radiograph analysis device includes a brightness relation information acquisition unit configured to acquire brightness relation information generated based on information related to a quantity of radiation and showing a relation between a brightness of the marker area and a brightness of a reference portion assumed to be a portion other than the marker, a reference brightness acquisition unit configured to acquire the brightness of the reference portion, and a marker area detection unit configured to detect the marker area based on the brightness relation information acquired by the brightness relation information acquisition unit and the brightness of the reference portion acquired by the reference brightness acquisition unit.
