Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Поволжский государственный технологический университет" (RU)
发明人:
Шейкина Ольга Викторовна (RU),Новиков Петр Сергеевич (RU),Шургин Алексей Иванович (RU)
申请号：
RU2013134048/10
公开号：
RU2013134048A
申请日:
2013.07.19
申请国别(地区):
RU
年份:
2015
代理人:
摘要：
1. A method for the identification of genotypes woody plant based markers comprising sampling each genotype under study, isolation of DNA from each genotype separately standard method, selection and synthesis of primers for the polymerase chain reaction, DNA amplification conducting alternately with each primer amplification products separation into fractions by gel electrophoresis, visualization of the amplification products in the rays of ultraviolet light, the determination of allele lengths for each genotype using standard marker selection etc. of beings for each genotype profile as its marker, wherein the DNA amplification is performed on the next set ISSR loci using primers: and from 55 to 65 ° C.2 annealing temperature of the primers. A method according to claim 1, characterized in that identification is carried out based on the markers that reflect variability DNA regions dispersed throughout genomu.3. A method according to claim 1, characterized in that the sampling of each genotype under study is conducted by drilling in trunk sterile drill cores 5-10 5-10 mm diameter to a depth of 1-1.5 see 4. A method according to claim 1, characterized in that the DNA extraction is carried out struzhki.5 wood. A method according to claim 1, characterized in that the produced synthesis primers constituting elements complementary to repetitive genomic oligonucleotides having arbitrary base pair at the 3 kontse.6. The method claim 1, characterized in that the polymerase chain reaction was carried out under the following conditions: pre-denaturation - 5 min 45 cycles: denaturation - 30 s primer annealing - 45 s, elongation - 2 min final elongation - 7 Min.7. The method of claim 1, wherein1. Способ идентификации генотипов древесных растений на основе маркеров, включающий отбор пробы каждого исследуемого генотипа, выделение ДНК из каждого генотипа отдельно стандартным методом, отбор и синтез праймеров для полимеразной цепной реакции, проведение амплификации ДНК поочередно с к
