PROBLEM TO BE SOLVED: To provide a biomaterial derived compound or a composition for prevention of disease injury caused by phytopathogenic fungi.SOLUTION: A method for production of a carbonic acid ester extract from angleworm fecal soil includes (A) a process to obtain a first aqueous phase by furnishing angleworm fecal soil to an aqueous extraction process, (B) a process to obtain an organic phase and a second aqueous phase by furnishing the first aqueous phase to an organic solvent extraction process, the solvent being other than carbonic acid ester shown by general formula (I), (C) a process to obtain a carbonic acid ester phase and a third aqueous phase by furnishing the second aqueous phase to an carbonic acid ester extraction process, the carbonic acid ester being shown by general formula (I): R-COOR (wherein R represents a 1-8C alkyl group, R represents a 1-4C alkyl group), and (D) a process to obtain a carbonic acid ester extract by removing the carbonic acid ester from the carbonic acid ester phase.COPYRIGHT: (C)2013,JPO&INPIT【課題】植物病原糸状菌による病害を防除する生体材料由来の化合物または組成物を提供する。【解決手段】(A)ミミズ糞土を水抽出工程に供して、第一水相を得る工程、(B)前記第一水相を、一般式(I)で表されるカルボン酸エステル以外の有機溶媒抽出工程に供して、有機相と第二水相とを得る工程、(C)前記第二水相を一般式(I):R-COOR’(Rは炭素数1~8のアルキル基、R’は炭素数1~4のアルキル基である)で表されるカルボン酸エステル抽出工程に供してカルボン酸エステル相と第三水相とを得る工程、および(D)前記カルボン酸エステル相からカルボン酸エステルを除去してカルボン酸エステル抽出物を得る工程、を含む、ミミズ糞土のカルボン酸エステル抽出物の製造方法。【選択図】図1