Frank Burroughs;Jayakumar P. Samuel;Narasimha C. Samboju;Kerrm Y. Yau;Steven R. Webb
发明人:
Narasimha C. Samboju,Kerrm Y. Yau,Frank Burroughs,Jayakumar P. Samuel,Steven R. Webb
申请号:
US13178199
公开号:
US08653327B2
申请日:
2011.07.07
申请国别(地区):
US
年份:
2014
代理人:
摘要:
Methods for introducing a linear nucleic acid molecule of interest into a cell comprising a cell wall include use of nanoparticles coated with polyethylene glycol. In some embodiments, the cell comprising a cell wall is a plant cell. Methods include genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall. Transgenic plants include a nucleic acid molecule of interest produced by regeneration of whole plants from plant cells transformed with linear nucleic acid molecules.