JI, YANSHAN,WANG, WENLING,HUANG, ER-TE JAMIE,MOORE, KAREN ELIZABETH,WHITE, KIMBERLY ANN
申请号：
CA2900922
公开号：
CA2900922A1
申请日:
2014.02.10
申请国别(地区):
CA
年份:
2014
代理人:
摘要：
A method for isolating genomic DNA (gDNA) from a biological material, the method including (a) contacting a sample that contains gDNA with a solution of hydroxide and a detergent to denature the gDNA； (b) mixing into the solution resulting from step (a) a solution characterized by high salt and reducing the pH of the solution to less than 1 0, thereby producing a neutralized solution； (c) centrifuging the sample to clarify the preparation； and (d) removing Insoluble material from the neutralized and clarified preparation, whereby a solution of gDNA is produced.
