Provided is an ultrasound probe including a bonded chemical barrier. The chemical barrier is manufactured between a matching layer and a lens layer by cross-sectionally applying a sputtering layer formed of a nichrome (NiCr) layer and a titanium (Ti) layer onto polyetherimide (PEI) to thereby completely prevent lifting of the lens layer or rising of the matching layer. In addition, as the chemical barrier is attached between the lens layer and the matching layer by using an epoxy adhesive, or a urethane adhesive, the ultrasound probe is fully resistant to long time soaking in an antiseptic, an abstergent, a gel, or the like.