Kiyotaka Iwasaki,Mitsuo Umezu,Koji Kojima,Charles Alfred Vacanti
申请号:
US12681049
公开号:
US08748166B2
申请日:
2008.10.10
申请国别(地区):
US
年份:
2014
代理人:
摘要:
A system (10) for forming and maintaining a biological tissue by which a biological tissue can be artificially formed by culturing cells, which comprises a pulse pump (12), a circulation pathway (13) having such a circuit structure as allowing a liquid cell culture medium discharged from the pulse pump (12) to return into the pulse pump (12), and a cell culture section (14A) and a gas exchange section (14B) provided along the circulation pathway (13). The cell culture section (14A) holds a cell holder (H) in such a manner to form a first channel wherein the liquid cell culture medium flowing in the circulation pathway (13) passes through the cell holder (H) and returns into the circulation pathway (13) and a second channel wherein the liquid cell culture medium flowing in the circulation pathway (13) passes outside the cell holder (H) and returns into the circulation pathway thereby bringing about a difference in pressure between the liquid cell culture medium passing through the respective channels.