The present invention relates to a nanoemulsion eyedrop composition and a method for preparing the same which increases the solubility of cyclosporine as an active ingredient and improves the stability of the eyedrop composition by mixing cyclosporine, a nonaqueous solvent, an emulsifier, and an aqueous solvent, and a method for preventing or treating dry eye syndrome using the same. The nanoemulsion eyedrop composition according to the present invention is characterized by having an average particle size of 200 nm or less, preferably 100 nm or less, and having a very narrow particle distribution. Therefore, the composition enables sterilizing filtration, improves stability, and has an excellent effect of clinically improving foreign body sensation and blurred vision, and thus can be effectively used for ophthalmic administration.
