Dispersing a gas in a liquid to provide a mixture of saturated, supersaturated or hypersaturated solution to provide a suspension of bubbles containing gas therein into which human tissue is immersed for treating same. A two-phase mixture is provided having a dissolved gas and a suspension of microbubbles in a liquid. Methods for making, maintaining, and using the two-phase mixture are provided. The gas molecules may be introduced into the liquid at high velocity under elevated pressure to form a supersaturated solution that retains the dissolved gas concentration in solution when exposed to ambient conditions.
