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СПОСОБ ПОЛУЧЕНИЯ ЛИЛИЙ, СОДЕРЖАЩИХ В ЛЕПЕСТКАХ ДЕЛФИНИДИН
专利权人:
САНТОРИ ХОЛДИНГЗ ЛИМИТЕД (JP)
发明人:
ТАНАКА Ёсикадзу (JP),НАКАМУРА Норико (JP),КОБАЯСИ Хитоси (JP),ОКУХАРА Хироаки (JP),КОНДО Масаёси (JP),КОЙКЭ Ёсукэ (JP),ХОСИ Ёсукэ (JP),НОМИДЗУ Тосикадзу (JP)
申请号:
RU2013117396/10
公开号:
RU2013117396A
申请日:
2011.09.16
申请国别(地区):
RU
年份:
2014
代理人:
摘要:
1. A method for producing lilies containing delphinidin in the petals, comprising the steps of: administering a lily isolated from bells gene flavonoid 3 , 5-hydroxylase (F35H), consisting of the nucleotide sequence shown in SEQ ID NO: 1 or SEQ ID NO: 11 or a nucleotide sequence identical to at least 90% nucleotide sequence shown in SEQ ID NO: 1 or SEQ ID NO: 11, which also encodes a polypeptide having F35H activity , administering the fragment isolated from gene lily flavonoid 3-hydroxylase (F3H), consisting present the nucleotide sequence shown in SEQ ID NO: 3 or SEQ ID NO: 16 or a nucleotide sequence identical to at least 90% nucleotide sequence shown in SEQ ID NO: 3 or SEQ ID NO: 16 which is also encodes a polypeptide having an activity of F3H, and delphinidin synthesis as a result of the introduced gene F35H, wherein the suppression of endogenous gene expression F3H, which is involved in the synthesis of cyanidin in the petals liliy.2. A method according to claim 1, wherein the suppression of expression of an endogenous F3H carried RNAL3 manner. A method according to any of claims 1 or 2, wherein the F35H gene is isolated from the bell consists of a nucleotide sequence identical to at least 95% nucleotide sequence represented by SEQ ID NO: 1 or SEQ ID NO: 11 which also encode a polypeptide having an activity F35H.4. A method according to claim 3, wherein the bell isolated from F35H gene consists of the nucleotide sequence shown in SEQ ID NO: 1 or SEQ ID NO: 11.5. A method according to any of claims 1 or 2, wherein the fragment isolated from lily F3H gene consists of the nucleotide sequence,1. Способ получения лилий, содержащих в лепестках делфинидин, включающий следующие стадии:введение в лилии выделенного из колокольчиков гена флавоноид-3,5-гидроксилазы (F35H), состоящего из нуклеотидной последовательности, представленной в SEQ ID NO:1 или SEQ ID NO:11, или нуклеотидной последовательности, идентичной, по меньшей мере, на 90% нуклеотидной последовательности, пр
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