A guide puncturing needle which is integratively used with a scanning and detecting device, such as CT, MRI or the like, and provides puncturing, biopsy, injection, implanting, and the physical diagnose and therapy, such as RF, microwave, freezing, laser and the like, and a puncturing guide method for applying the puncturing needle to puncture are provided. Since the guide puncturing needle is provided with a needle-entering angle guide means (2), a puncturing layer levelling component (31), a needle-entering reference line levelling component (32), whether the CT gantry and the MRI scanning layer are at any angle, the actual needle-entering layer is always adjusted to just superpose to the CT scanning layer, the actual needle-entering angle is always just the same as the needle-entering angle needed for the scanning and detecting device, thus allow the puncturing needle to arrive at the focus point exactly accurately.
