Decreasing lactate level and increasing polypeptide production by downregulating the expression of lactate dehydrogenase and pyruvate dehydrogenase kinase
Meixia Zhou,Bradley Richard Snedecor,Chi Kin Domingos Ng,Amy Shen
申请号：
US13688075
公开号：
US09487809B2
申请日:
2012.11.28
申请国别(地区):
US
年份:
2016
代理人:
摘要：
The present invention provides methods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for a LDH and an siRNA specific for a PDHK.
