A biological information acquisition apparatus includes a light source, a light-receiving unit, and a calculation unit. The light source is configured to irradiate a biological body with light. The light-receiving unit is configured to receive the light from the biological body. The calculation unit is configured to determine biological information. The calculation unit is configured to detect a first value by using a first signal from the light-receiving unit while the light source emits the light, detect a second value by using a second signal from the light-receiving unit while the light source does not emit the light, determine a temperature of the biological body based on the second value, and correct the first value using the temperature.