The present invention provides methods to identify acceptor germline sequences for a humanized antibody, which methods comprise the steps of: a) identifying a non-human antibody that has the desired biological activity； b) determining the amino acid sequence of a non-human antibody V H and V L domains； and c) comparing the nonhuman antibody sequence to a group of human germline sequences, wherein the comparison comprises the substeps of: (1) assigning the sequence of non-human V H and V L domain sequences residue numbers； (2) delineating the CDR and FR regions in the sequence； (3) assigning a predetermined numerical score at each residue position for which the non-human and human germline sequences are identical； and (4) totaling all of the residue scores to generate a total score for each human germline sequence； and d) identifying the human germline sequence with the highest total residue score as the acceptor germline sequence.본 발명에서 제공된 방법 및 조성물은 일반적으로 IL-10 특이적 항체 및 이의 용도에 관한 것이다. 더욱 상세하게는, 사람화된 IL-10 특이적인 항체의 조성물 및 IL-10의 생물학적 활성의 조절, 특히 자가면역 질환 및 병원체-매개된 면역병리학에 있어서 이러한 항체의 사용 방법에 관한 것이다.
