The present invention provides a reducing cholesterol probiotic strain, composition thereof and use thereof. The probiotic strain is Lactobacillus plantarum, which itself and its metabolites can effectively reduce the cholesterol content, enhance the gene expression level of SCARB1, APOA1, and LDLR to promote the effect of cholesterol metabolism, and can effectively reduce the total cholesterol and the low-density lipoprotein in human blood.
