Provided is a biological observation apparatus including: illuminating portions that irradiate biological tissue with illumination light including light in R, G, and B regions, respectively； an image acquisition portion that acquires image signals from reflected light of the illumination light coming from the biological tissue； narrow-band-light generating portions that are disposed in the illuminating portions or the image acquisition portion and that, in wavelength bands of the illumination light, generate two narrow-band beams for at least one of the R, G, and B wavelength bands constituting the illumination light, on either side of a central wavelength of that wavelength band； and an image-generating portion that generates an image on the basis of two or more types of the image signals obtained from the reflected light including two or more narrow bands acquired by the image acquisition portion.
