PROBLEM TO BE SOLVED: To provide a method for inducing the differentiation of human tooth pulp cells into odontoblasts, by which dental disease patients such as dental caries patients can be treated, and to provide a method for regenerating a dentin.SOLUTION: This method for inducing the differentiation of human tooth pulp cells into odontoblasts comprises three-dimensionally culturing at least one of separated human tooth pulp cells or cells capable of being differentiated into the human tooth pulp cells in a high density in the presence or absence of a carrier, while adding 1,25-(dihydroxy)vitamin D3, and the method for regenerating the dentin.COPYRIGHT: (C)2006,JPO&NCIPI【課題】 う蝕などの歯科疾患患者を治療することを可能にするヒト歯髄細胞の象牙芽細胞への分化誘導方法および象牙質の再生方法を提供すること。【解決手段】 分離したヒト歯髄細胞又はヒト歯髄細胞に分化可能な細胞のうち少なくとも1種類を担体の存在または非存在下、1,25(ジヒドロキシ)ビタミンD3を添加しながら三次元的に高密度で培養することによる象牙芽細胞への分化誘導方法及び象牙質の再生方法。【選択図】 なし
