The present invention relates to a method of selecting and separating normal cells and specific cells by using ultrasonic waves. More particularly, the present invention relates to a method of selecting and separating normal cells and cancer cells, or normal cells and stem cells by using a difference in attenuation coefficients, speed of sound and so on in normal cells and specific cells obtained when ultrasonic waves are radiated. The method of the present invention is capable of selecting and separating normal cells and specific cells (cancer cells or stem cells) in a simple and efficient manner.