Provided herein are systems and methods to determine vaccine potency and toxin concentration. The provided systems and methods may be multiplexed to determine potency and toxin concentration simultaneously. The systems and methods may be microarray based, providing accurate results while reducing the amount of testing time required compared to current potency and toxin concentration tests which often require the use of animal subjects or expensive test materials. Further, the provided systems and methods may detect desired antigens, endotoxins and exotoxins.
