A specimen retrieval device for extraction of tissue includes two ports and a specimen receptacle affixed to a support assembly at a distal portion of an inner shaft assembly that passes through the two ports. The first port has a larger diameter than the second port, so that introduction of the second port into the longitudinal bore of the first port introduces the specimen receptacle into a patient's body. In embodiments, the specimen receptacle has two openings which may be used to transfer a tissue specimen from within a patient's body cavity to outside of the patient's body.
