PROCEDES SUSCEPTIBLES D'ETRE DEVELOPPES POUR LA PRODUCTION D'UN VECTEUR VIRAL ADENO-ASSOCIE (AAV) DANS UN SYSTEME DE CULTURE CELLULAIRE EN SUSPENSION EXEMPT DE SERUM APPROPRIE POUR UNE UTILISATION CLINIQUE SYSTEME

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专利权人:

INC.;SPARK THERAPEUTICS

发明人:

QU, GUANG,LU, LIN,WRIGHT, JOHN FRASER

申请号：

CA3006309

公开号：

CA3006309A1

申请日:

2016.12.01

申请国别(地区):

CA

年份:

2017

代理人:

摘要：

Methods and compositions for transfecting cells with plasmids are disclosed. In certain embodiments, methods and compositions are disclosed in which transfection efficiency is significantly increased by contacting the cells being transduced with polyethyleneimine (PEI) that is free of nucleic acid during the transfection process. Therapeutically useful adeno-associated viral vectors generated according to the disclosed methods and compositions are also disclosed.

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中国工程科技知识中心

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http://www.ckcest.cn/home/