The present invention provides a surgical navigation system including a positioning device, a processing device and a display device. The positioning device includes an auxiliary positioning assembly capable of wearing or fixing around a patient's affected part and an optics positioning assembly. The optics positioning assembly can sense the position of the auxiliary positioning assembly to form a positioning information. The processing device can receive the positioning information and integrate the positioning information with a medical image to form a navigation information, so that the navigation information is displayed on the display device through a stereoscopic image or a sectional image. Therefore, the doctor can accurately perform the relevant surgical operations on the patient's affected part through the displayed information.