Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences
发明人:
GAO, Caixia,JI, Xiang,ZHANG, Huawei
申请号：
EP20160761120
公开号：
EP3309255(A1)
申请日:
2016.03.14
申请国别(地区):
欧洲专利局
年份:
2018
代理人:
摘要：
The present invention discloses a method for improving the ability to resist against an intrusive DNA virus of a plant. The present invention provides a method for making a plant with improved ability to resist against a DNA virus, specifically can comprising the following steps: 1) selecting a sequence comply with 5'-N X -NGG-3' or 5'-CCN-N X -3' from the genomic sequence of the DNA virus as the target sequence; designing a DNA sequence reverse-complementary to said target sequence; 2) constructing said DNA sequence to a vector for expressing CRISPR/Cas9 nuclease so as to obtain a recombinant vector capable of transcribing a guide RNA and expressing a Cas9 protein; the crRNA in said guide RNA contains a RNA fragment transcribed from said DNA fragment; 3) introducing said recombinant vector into a recipient plant so as to obtain a plant with improved ability to resist against said DNA virus. The method of the invention merely depends on the genomic sequence of the virus, without the need of knowing specific f
