PROBLEM TO BE SOLVED: To provide a highly biotin-containing yeast which contains biotin absorbed in high concentration and little has the odor of the yeast, and to provide a method for producing the same, especially a method for producing the highly biotin-containing yeast, comprising finding culture conditions for inhibiting the proliferation of yeast and simultaneously remarkably absorbing the biotin, and then culturing the yeast under the conditions.SOLUTION: This method for producing the highly biotin-containing yeast is characterized by dissolving biotin in a solvent, preferably an alkaline solvent, adding the solution to a carbon source-free yeast culture solution, adjusting the pH of the yeast culture solution on the acidic side, and then culturing the yeast culture solution at a high temperature.COPYRIGHT: (C)2008,JPO&INPIT【課題】本発明が解決しようとする課題は、高濃度のビオチンが吸収され、かつ酵母臭の少ないビオチン高含有酵母及びその製造方法を提供すること。特に、酵母増殖を抑えつつ顕著にビオチンが吸収される培養条件を見出し、その条件下にて酵母を培養しビオチン高含有酵母を製造する方法を提供すること。【解決手段】ビオチンを溶媒好ましくはアルカリ性の溶媒に溶解させ、それを炭素源を含まない酵母培養液に添加し、酵母培養液のpHを酸性側にし、酵母培養液を高温度にして培養することを特徴とする、ビオチン高含量酵母の製造方法。【選択図】なし
