培養重層上皮シートの作製方法
- 专利权人:
- 国立大学法人富山大学
- 发明人:
- 二階堂 敏雄,岡部 素典,齋藤 滋,倉科 憲治,小池 剛史
- 申请号：
- JP2007040378
- 公开号：
- JP5181172B2
- 申请日:
- 2007.02.21
- 申请国别(地区):
- JP
- 年份:
- 2013
- 代理人:
- 摘要：
PROBLEM TO BE SOLVED: To provide a method for producing a cultured multilayer epithelium sheet using a dried amnionic membrane produced by a specific drying method.
SOLUTION: A dried amnionic membrane keeping the cellular tissue of a raw amnionic membrane is obtained by continuously heating a raw amnionic membrane placed in a processing tank by a far-infrared heater provided in the processing tank, pressure-reducing operation to decrease pressure in the processing tank, keeping to warm the raw amnionic membrane by irradiating a microwave from a microwave heating device provided outside of the processing tank, pressure-recovering operation to recover the pressure of the processing tank under reduced pressure, and repeating the pressure-reducing or recovering operation in several times. The obtained amnionic membrane itself has the functions of a scaffold and feeder cell, and is useful for producing the cultured multilayered epithelium sheet.
COPYRIGHT: (C)2008,JPO&INPIT
- 来源网站：
- 中国工程科技知识中心
