Disclosed is a method for processing sorted sperm cells compromised by a sorting event comprising the steps of: subjecting a plurality of sperm cells to sorting stresses； selecting said plurality of sperm cells for a desired characteristic； providing a protein-free sperm cell extender； providing a protein-free cryoprotectant-containing sperm cell extender； combining said protein-free sperm cell extender and said protein-free cryoprotectant-containing sperm cell extender to form a cryoprotectant- containing centrifugation medium； maintaining said cryoprotectant-containing centrifugation medium in an unclarified state； adding said plurality of sperm cells subjected to soiling stresses and selected for a desired characteristic to said unclarified cryoprotectant- containing centrifugation medium to form an unclarified cryoprotectant- containing sperm cell centrifugation medium； subjecting said unclarified cryoprotectant-containing sperm cell centrifugation medium to centrifugation； and decanting a portion of said centrifuged unclarified cryoprotectant- containing sperm cell centrifugation medium.
