An electrochemical-based analytical test strip for the determination of an analyte (such as glucose) in a bodily fluid sample (for example, a whole blood sample) and/or a characteristic of the bodily fluid sample (for example, hematocrit) includes a first sample-receiving chamber with first and second sample-application openings, and first and second electrodes. The first and second electrodes are disposed in the first sample-receiving chamber between the first and second sample-application openings. The electrochemical-based analytical test strip also includes a second sample-receiving chamber and a plurality of electrodes disposed in the second sample-receiving chamber. In addition, the second sample-receiving chamber intersects the first sample-receiving chamber between the first and second electrodes, thereby defining a chamber intersection.一種用於測定一體液取樣(例如一全血取樣)中之一分析物(如葡萄糖)及/或該體液取樣之一特徵(例如血容比)之基於電化學的分析試驗帶,其包括一第一取樣接受腔,該第一取樣接受腔具有第一及第二加樣口和第一及第二電極。該第一及第二電極係配置於該第一取樣接受腔中且位於該第一及第二加樣口之間。該基於電化學的分析試驗帶亦包括一第二取樣接受腔及複數個配置於該第二取樣接受腔中之電極。此外,該第二取樣接受腔與該第一取樣接受腔在該第一及第二電極之間交叉,藉此定義一腔交集區。110‧‧‧電絕緣基材層120‧‧‧圖案化導體層130‧‧‧酶試劑層140‧‧‧圖案化間隔物層150‧‧‧親水性層160‧‧‧頂層162‧‧‧第一取樣接受腔164‧‧‧第二取樣接受腔166‧‧‧第一加樣口168‧‧‧第二加樣口170‧‧‧腔交集區