The invention provides a method and system for non-invasive analysis. In particular the inventive system relates optical techniques involving infra-red, visible or ultra violet radiation for imaging and analyzing surface and sub-surface structure of tissue and components of the eye. Embodiments of the invention further include Optical Coherence Tomography (OCT) techniques for sub-surface imaging and analysis, and incorporate instruments such as ophthalmoscopes, fundus cameras, for non-invasive imaging and analysis.