MAX-PLANCK-GESELLSCHAFT ZUR FÖRDERUNG DER WISSENSCHAFTEN E.V.;UNIVERSITY OF MASSACHUSETTS;WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH;MASSACHUSETTS INSTITUTE OF TECHNOLOGY
发明人:
TUSCHL, THOMAS,SHARP, PHILLIP A,BARTEL, DAVID P,ZAMORE, PHILIP D
申请号：
DK10184711
公开号：
DK2345742T3
申请日:
2001.03.30
申请国别(地区):
DK
年份:
2014
代理人:
摘要：
Double-stranded RNA (dsRNA) induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). Using a Drosophila in vitro system, we demonstrate that 19-23 nt short RNA fragments are the sequence-specific mediators of RNAi. The short interfering RNAs (siRNAs) are generated by an RNase III-like processing reaction from long dsRNA. Chemically synthesized siRNA duplexes with overhanging 3' ends mediate efficient target RNA cleavage in the lysate, and the cleavage site is located near the center of the region spanned by the guiding siRNA. Furthermore, we provide evidence that the direction of dsRNA processing determines whether sense or antisense target RNA can be cleaved by the produced siRNP complex.
