The present disclosure relates to a method of injecting objects, such as eggs, with a fluid, wherein said objects each comprise at least two object parts, such embryo tissue, embryotic or allantoic fluid and an air pocket, of which at least one object part is to be injected, by advancing injection needles to a determined depth, which depth is expected to correspond with the at least one object part to be injected, into the objects and injecting fluid into the objects at the determined injection depth. A corresponding injection system is disclosed. Also, an independent calibration mechanism/algorithm is disclosed.