ДЖУЛИАНИ Джаммария (IT),БЕНЕДУЗИ Анна (IT),ГОБЕТТИ Марко (IT),Дль КАНЬО Раффаэлла (IT),ДЕ АНДЖЕЛИС Мария (IT),КАЛАССО Мария (IT)
申请号：
RU2012134407/10
公开号：
RU2012134407A
申请日:
2011.01.04
申请国别(地区):
RU
年份:
2014
代理人:
摘要：
1. Biotechnological method for the synthesis of biomass containing or consisting of at least one plantaricin selected from plantaricins A, K or N, or mixtures thereof and lactic acid bacteria Lactobacillus plantarum DSM 23213 and Lactobacillus rossiae DSM 23214 or for the preparation of one or more plantaricins, selected from plantaricins A, K or N, or mixtures thereof, which includes or consists of: a) culturing lactic acid bacteria Lactobacillus plantarum DSM 23213 and Lactobacillus rossiae DSM 23214； b) co-inoculating a substrate selected from the group consisting of CDM, WFH, grape must, milk th serum or extracts of fruit and vegetable products, an aqueous suspension of lactic acid bacteria, as in a)；. c) incubating； and, if necessary, d) centrifuging the culture medium to remove lactic acid bacteria cells. 2. The method according to claim 1, wherein the suspension from step a) has a cell density of approximately 9.0 Log CFU / ml for each type of lactic acid bacteria and is added to the substrate as a percentage of the volume of the substrate from 1 to 4%. The method according to claim 1, in which the incubation was carried out at temperatures from 30 to 37 ° C, preferably 30 ° C, for 18-24 hours, preferably 18 hours. The method according to claim 1, in which centrifugation was performed at 10,000 x g for 15 min at 4 ° C. A method according to any one of claims 1 to 4, comprising step e) for dehydrating the supernatant obtained in step d) by drying or freeze drying. Biomass obtained or obtained using the method according to any one of claims 1 to 5, containing or consisting of at least one plantaricin selected from plantaricins A, K or N or mixtures thereof and lactic acid bacteria Lactobacillus plantarum DSM 23213 and Lactobacillus rossiae DSM 23214.7 . Pharmaceutical1. Биотехнологический способ синтеза биомассы, содержащей или состоящей из по меньшей мере одного плантарицина, выбранного из плантарицинов А, K или N, или их смесей и молочнокислых бактерий Lactob
