The present disclosure provides a laser guided positioning device and method for an automatic tumor puncture machine. Three-dimensional images of a patient on a puncture sickbed are collected by using three-dimensional cameras, a CT image is collected, metal sheets are disposed as identification points, a three-dimensional point cloud of the patient is reconstructed according to the collected three-dimensional information, a three-dimensional point cloud space coordinate system corresponding to the CT image is established according to the metal identification points placed on the patient in advance, the coordinate position of a puncture point in the three-dimensional point cloud is determined according to a puncture path pre-designed in the CT image, and laser guidance is performed according to the determined puncture point.
