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БИСПЕЦИФИЧЕСКИЕ АНТИТЕЛА
专利权人:
РОШ ГЛИКАРТ АГ
发明人:
БРИНКМАНН Ульрих,КРОАСДАЛЕ Ребекка,ДЮРР Харальд,КЛЯЙН Кристиан,КОПЕЦКИ Эрхард,ЛАУ Вильма,РЕГУЛА Йорг Томас,ШАНЦЕР Йюрген Михель,УМАНА Пабло,ВАРТА Катарина
申请号:
RU2012143798
公开号:
RU2012143798A
申请日:
2011.03.24
申请国别(地区):
RU
年份:
2014
代理人:
摘要:
1. A bispecific antibody soderzhascheea) heavy chain and light chain of a first full-length antibody that specifically binds to a first antigen b) a heavy chain and a light chain of the second full-length antibody that specifically binds to a second antigen, where the N-end of the heavy chain linked a light chain C-terminus via a peptide linker.2. The antibody of claim 1, characterized in chtoSN3-domain of an antibody heavy chain and full length) and CH3 domain of an antibody heavy chain full length b) occur at the contact surface, which includes changes in the initial contact surface of the antibody CH3 domains wherein i) in the CH3 domain of one heavy tsepiaminokislotny residue is replaced with an amino acid residue with a larger side chain volume and thereby creating a projection of the contact surface of the CH3 domain of one heavy chain that is placed in the cavity on the contact surface of the CH3 domain of another cha heavy chain and gdeii) in the CH3 domain of the other heavy tsepiaminokislotny residue is replaced with an amino acid residue side chain of a smaller volume and thus creates a cavity in the contact surface of the second CH3 domain, wherein the protrusion is located on the contact surface of the first CH3 domena.3. An antibody according to claim 2, characterized in chtoukazanny amino acid residue having a larger side chain volume is selected from the group consisting of arginine (R), phenylalanine (F), tyrosine (Y), tryptophan (W), iukazanny amino acid residue having smaller side chain volume is selected from the group consisting of alanine (A), serine (S), threonine (T), valine (V) .4. An antibody according to claim 2, characterized in chtooba CH3 domain further modified by the introduction of cysteine ​​(C) QUALITY1. Биспецифическое антитело, содержащееа) тяжелую цепь и легкую цепь первого антитела полной длины, которое специфически связывается с первым антигеномб) тяжелую цепь и легкую цепь второго антитела полной длины, которое специфически с
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