KIM, HYO SOO,김효수,YANG, HAN MO,양한모,KIM, JU YOUNG,김주영,LEE, JOO EUN,이주은,KIM, HYO SOOKR,YANG, HAN MOKR,KIM, JU YOUNGKR,LEE, JOO EUNKR
申请号：
KR1020170103712
公开号：
KR1020180020906A
申请日:
2017.08.16
申请国别(地区):
KR
年份:
2018
代理人:
摘要：
The present inventors have studied an example in order to enhance the differentiation efficiency and maturation of cardiomyocytes derived from stem cells. As a result, it has been confirmed that the differentiation efficiency into stem cell-based cardiomyocytes is improved in an NecroX treatment group, and that further matured cardiomyocytes can be obtained in a short period of time, and the present invention has been completed. Through the treatment of NecroX according to the present invention, cardiomyocytes derived from stem cells have a large size and great thickness compared to a control group, and a strong and dense actin structure is shown. Therefore, it is shown that cardiomyocytes derived from stem cells induced by NecroX treatment can be produced with improved maturation. Accordingly, high efficiency and high purity differentiation of cardiomyocytes derived from stem cells can be induced when NecroX is treated. According to the present invention, NecroX, as a useful material capable of improving the differentiation efficiency and maturation of other differentiated cells, secures differentiation of cardiomyocytes in patient-customized induced pluripotent stem cells, and thus is expected to be helpful in producing a cardiac disease model.본 발명자들은 줄기세포 유래 심근세포의 분화 효율을 증진시키고 성숙도를 향상시키기 위해 예의 연구한 결과, 네크로엑스(NecroX) 처리군에서 줄기세포기반 심근세포로의 분화효율이 증가하였을 뿐만 아니라 더 성숙한 심근세포를 단기간에 획득할 수 있다는 사실을 확인하고, 본 발명을 완성하였다. 본 발명에 따른 NecroX의 처리로 줄기세포 유래 심근세포는 세포의 크기가 대조군에 비해 크고 굵으며, 튼튼할 뿐만 아니라 치밀한 액틴의 구조를 보여, NecroX 처리로 유도된 줄기세포유래 심근세포는 성숙도가 높아진 심근세포를 제작할 수 있음을 보여주는바, 따라서 NecroX를 처리하면 줄기세포에서 유래된 심근세포의 고효율, 고순도 분화를 유도할 수 있다. 이에, 본 발명의 NecroX는 다른 분화세포의 분화효율과 성숙도도 증가시킬 수 유용한 물질로서 환자 맞춤형 유도만능줄기세포에서 심근세포의 분화를 확립해 심장질환 모델을 제작하는데 도움이 될 것으로 기대된다.
