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Método para transformación de mitocondria de células vegetales
专利权人:
Algentech SAS
发明人:
MALCUIT, Isabelle,SOROKIN, Alexander
申请号:
ES09795513
公开号:
ES2611656T3
申请日:
2009.11.25
申请国别(地区):
ES
年份:
2017
代理人:
摘要:
A method for transforming a plant cell, the method comprises: 1) introducing into the nucleus of said plant cell a first nucleic acid sequence comprising a plant nuclear promoter operably linked to a first nucleic acid sequence comprising a transgene cassette of plant mitochondria, a sequence of translocation of plant mitochondria is an RNA sequence capable of being bound to a binding protein translocation sequence of plant mitochondria, and a binding domain primer selected from that of a retrotransposon or retrovirus 2) introducing into the nucleus of said plant cell a second nucleic acid sequence encoding a binding protein sequence fused translocation to a transit peptide of plant mitochondria, wherein said second nucleic acid sequence is operably linked to a nuclear plant promoter and 3) introducing into the nucleus of said plant cell a third nucleic acid sequence encoding a fused reverse transcriptase protein to a transit peptide of plant mitochondria, wherein the third nucleic acid sequence is operably linked to a plant nuclear promoter which drives expression in a plant cell nucleus wherein the transgene cassette of plant mitochondria comprising: i) a sequence flanking left and right flanking sequence selected from nucleotide sequences used for homologous recombination in mitochondria and ii) at least one isolated nucleic acid of interest.Un método para transformar una célula vegetal, el método comprende: 1) introducir en el núcleo de dicha célula vegetal una primera secuencia de ácidos nucleicos que comprende un promotor nuclear vegetal ligado operablemente a una primera secuencia de ácidos nucleicos que comprende un casete de transgén de mitocondria vegetal, una secuencia de translocación de mitocondrias vegetales que es una secuencia de ARN capaz de ser unida a una proteína de unión de secuencia de translocación de mitocondrias vegetales, y un dominio de unión de cebador seleccionado de aquel de un retrotransposón o de un retrovirus 2) introducir en el núc
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/
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